Part III: Culture
diagram 5

NITRATE REDUCTION TEST FOR IDENTIFICATION OF M. tuberculosis
CRYSTALLINE REAGENT METHOD
Procedure

Add 0.2ml of sterile saline  to a screw-cap tube

   
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Use a sterile loop/spade to  emulsify two loopfuls/spadefuls of a 4-week old culture in the saline

   
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Add 2ml of the NaNo3 substrate

   
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Shake well and incubate  upright in a 37EC  water bath for 3 hours and remove

   
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Use the tip of a spatula to  add a small amount of crystalline reagent to the test solution.  The quantity of reagent is not critical

   
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Examine immediately for a  pink to red colour and compare to colour standard

CONTACTS:

Dr Martie van der Walt
E-mail: vdwalt@mrc.ac.za

Dr Roxanna Rustomjee
E-mail: roxanna.rustomjee@
mrc.ac.za

Prof Valerie Mizrahi
E-mail: mizrahiv@
pathology.wits.ac.za

Prof. Paul van Helden
E-mail: pvh@sun.ac.za

 

Last updated:
22-Jun-2011

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