CULTURE Part III

Lab services in tuberculosis control
CULTURE Part III

Preface
Introduction
Laboratory layout and equipment
- Plan of a culture laboratory
- Arranging equipment and materials
- Care and maintenance of essential equipment
Specimen collection
- Containers
- Collection procedures
- Sputum specimens
- Other specimens
Specimen storage and transport
Specimen handling
- Receipt of incoming specimens
- Safe handling of specimens
Homogenisation and decontamination
- Digestion and decontamination procedures
- Sputum specimens
- Other specimens
Culture media
- Advantages and disadvantages of egg-based media
- Precautions during media preparation
- Preparation of egg-based media
- Löwenstein-Jensen medium
- Ogawa medium
- Acid-buffered Ogawa medium
Inoculation and incubation procedures
- Inoculation procedures
- Incubation of cultures
Culture examination and identification
- Examination schedule
- Reading of cultures
- Differentiation of M. tuberculosis
- Niacin test
- Nitrate reduction test
- Catalase test
- Growth on medium containing p-Nitrobenzoic acid (PNB)
Recording and reporting of laboratory results
Quality control
Selected references
- Diagrams

ANNEXES and FIGURES
available from Karin Weyer, E-mail: karin.weyer@mrc.ac.za, Tel: +27 12 3398551

CONTACTS:

Dr Karin Weyer
Dr Martie van der Walt
E-mail: vdwalt@mrc.ac.za
Dr Roxanna Rustomjee
E-mail: roxanna.rustomjee@
mrc.ac.za
Prof Valerie Mizrahi
E-mail: mizrahiv@
pathology.wits.ac.za
Prof. Paul van Helden
E-mail: pvh@sun.ac.za

Last updated:
22-Jun-2011

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