MICROSCOPY Part II

Lab services in tuberculosis control
MICROSCOPY Part II

Preface
Introduction
Laboratory layout and equipment
- Plan of a peripheral microscopy laboratory
- Arranging equipment and materials
- Care and maintenance of essential equipment
Specimen collection Containers
- Collection procedures
Specimen storage and transport
Specimen handling
- Receipt of incoming specimens
- Safe handling of specimens
- Evaluation of sputum quality and volume
Smear preparation procedures
- Sputum smear preparation
- Value of smears on extra-pulmonary specimens
Acid-fast staining procedures
- Ziehl-Neelsen staining
- Fluorochrome staining
- Precautions during staining
Smear examination procedures
- Components of the microscope
- Use of the microscope
- Examination procedures
- Morphological characteristics of acid-fast bacilli
- Causes or error in microscopy
- Consequences of false-positive and false-negative smears
Recording and reporting of results
- Negative
- Positive
- Quantification of Ziehl-Neelsen smear results
- Quantification of fluorochrome smear results
Quality control
Selected references
- Diagrams

ANNEXES and FIGURES
available from Karin Weyer, E-mail: karin.weyer@mrc.ac.za, Tel: +27 12 3398551

CONTACTS:

Dr Martie van der Walt
E-mail: vdwalt@mrc.ac.za
Dr Roxanna Rustomjee
E-mail: roxanna.rustomjee@
mrc.ac.za
Prof Valerie Mizrahi
E-mail: mizrahiv@
pathology.wits.ac.za
Prof. Paul van Helden
E-mail: pvh@sun.ac.za

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Last updated:
22-Jun-2011

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